Plant Transcription Factor Database
Previous version: v3.0
Arabis alpina
HSF Family
Species TF ID Description
KFK22685.1HSF family protein
KFK24450.1HSF family protein
KFK24845.1HSF family protein
KFK25539.1HSF family protein
KFK25540.1HSF family protein
KFK27750.1HSF family protein
KFK27929.1HSF family protein
KFK28556.1HSF family protein
KFK29528.1HSF family protein
KFK30320.1HSF family protein
KFK31692.1HSF family protein
KFK31930.1HSF family protein
KFK34438.1HSF family protein
KFK35490.1HSF family protein
KFK37011.1HSF family protein
KFK39761.1HSF family protein
KFK40020.1HSF family protein
KFK41216.1HSF family protein
KFK44645.1HSF family protein
KFK45015.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645